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anti igf2bp2  (Proteintech)


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    Structured Review

    Proteintech anti igf2bp2
    Anti Igf2bp2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+igf2bp2/IGF2BP2+Antibody/pm41925955-104-23-28
    Average 96 stars, based on 261 article reviews
    anti igf2bp2 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: METTL3 inhibition alleviates neuroinflammation and apoptosis by reducing ETV4 m6A modification
    Article Snippet: Antibodies included anti-METTL3 (ABclonal, A19079), anti-METTL14 (ABclonal, A8530), anti-WTAP (ABclonal, A14695), anti-FTO (ABclonal, A3861), anti-CD45 (Servicebio, GB113886 ), antiCD11b (Servicebio, GB15058), anti-c-Jun (Proteintech, 11515-1-AP), anti-Caspase3 (Servicebio, GB11767C), anti-Cleaved-Caspase-3 (Servicebio, GB115733 ), anti-GAPDH (Servicebio, GB15004), anti-p65 (Servicebio, GB11997), anti-p-p65 (Servicebio, GB113882 ), anti-ETV4 (proteintech, 10684-1-AP), anti-IGF2BP2 (proteintech, 11601-1-AP). collagenase IV were purchased from Sigma-Aldrich (9001-12-1).

    Western Blot:

    Article Title: m6A reader IGF2BP2 mediates paclitaxel resistance in esophageal squamous cell carcinoma via FOXM1 mRNA stabilization
    Article Snippet: Protein samples (30 μg) were separated by SDS-PAGE on 10% gels (cat. no. PG112; Shanghai EpiZyme Co., Ltd.) and then transferred onto PVDF membranes (cat. no. WGPVDF22; Servicebio Co., Ltd.). .. Subsequently, the membranes were blocked with high-efficiency western blocking reagent (cat. no. GF1815; Shanghai Genefist Co., Ltd.) for 15 min at room temperature and maintained overnight at 4°C with the primary antibodies, including anti-IGF2BP2 (1:2,000; cat. no. 11601-1-AP; Proteintech Group, Inc.), anti-FOXM1 (1:2,000; cat. no. 13147-1-AP; Proteintech Group, Inc.) and anti-GAPDH (1:5,000; cat. no. 10494-1-AP; Proteintech Group, Inc.). .. After rinsing the membrane three times with TBST (cat. no. G2150-1L; Wuhan Servicebio Technology Co., Ltd.), the PVDF membranes were exposed to secondary rabbit antibodies (1:5,000; cat. no. SA00001-2; Proteintech Group, Inc.) for 1 h at ambient temperature.

    Blocking Assay:

    Article Title: m6A reader IGF2BP2 mediates paclitaxel resistance in esophageal squamous cell carcinoma via FOXM1 mRNA stabilization
    Article Snippet: Protein samples (30 μg) were separated by SDS-PAGE on 10% gels (cat. no. PG112; Shanghai EpiZyme Co., Ltd.) and then transferred onto PVDF membranes (cat. no. WGPVDF22; Servicebio Co., Ltd.). .. Subsequently, the membranes were blocked with high-efficiency western blocking reagent (cat. no. GF1815; Shanghai Genefist Co., Ltd.) for 15 min at room temperature and maintained overnight at 4°C with the primary antibodies, including anti-IGF2BP2 (1:2,000; cat. no. 11601-1-AP; Proteintech Group, Inc.), anti-FOXM1 (1:2,000; cat. no. 13147-1-AP; Proteintech Group, Inc.) and anti-GAPDH (1:5,000; cat. no. 10494-1-AP; Proteintech Group, Inc.). .. After rinsing the membrane three times with TBST (cat. no. G2150-1L; Wuhan Servicebio Technology Co., Ltd.), the PVDF membranes were exposed to secondary rabbit antibodies (1:5,000; cat. no. SA00001-2; Proteintech Group, Inc.) for 1 h at ambient temperature.

    Article Title: TNF-α-driven m6A modification disrupts the immunoregulatory function of MSCs by regulating HDAC5-dependent super-enhancers.
    Article Snippet: SDS‒PAGE was used to separate protein extracts, which were subsequently transferred to 0.45 μm PVDF membranes (Millipore, MA, USA). .. After 1 h of blocking with 5% nonfat milk at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies, including anti-LIF (ab138002, Abcam), antiHDAC5 (ab55403, Abcam), anti-METTL3 (ab195352, Abcam), anti-WTAP (ab195380, Abcam), anti-METTL14 (ab309096, Abcam), anti-ALKBH5 (ab195377, Abcam), anti-FTO (ab126605, Abcam), anti-YTHDF2 (24744-1- AP, Proteintech), anti-YTHDC2 (27779-1-AP, Proteintech), anti-BRD2 (ab139690, Abcam), anti-BRD3 (ab50818, Abcam), anti-BRD4 (ab128874, Abcam), anti-IGF2BP1 (ab184305, Abcam), anti-IGF2BP2 (11601-1-AP, Proteintech), anti-IGF2BP3 (ab177477, Abcam), anti-YTHDF1 (ab252346, Abcam), anti-YTHDF3 (ab 220161, Abcam),anti-ACTIN(3700S, CST) and anti-GAPDH(5174S, CST). .. After 3 washes in PBST, the PVDF membranes were incubated with HRP-conjugated goat anti-mouse/rabbit secondary antibodies (1:3000, Boster, China) for 1 h at room temperature.

    Article Title: TNF-α-driven m6A modification disrupts the immunoregulatory function of MSCs by regulating HDAC5-dependent super-enhancers
    Article Snippet: SDS‒PAGE was used to separate protein extracts, which were subsequently transferred to 0.45 μm PVDF membranes (Millipore, MA, USA). .. After 1 h of blocking with 5% nonfat milk at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies, including anti-LIF (ab138002, Abcam), anti-HDAC5 (ab55403, Abcam), anti-METTL3 (ab195352, Abcam), anti-WTAP (ab195380, Abcam), anti-METTL14 (ab309096, Abcam), anti-ALKBH5 (ab195377, Abcam), anti-FTO (ab126605, Abcam), anti-YTHDF2 (24744-1-AP, Proteintech), anti-YTHDC2 (27779-1-AP, Proteintech), anti-BRD2 (ab139690, Abcam), anti-BRD3 (ab50818, Abcam), anti-BRD4 (ab128874, Abcam), anti-IGF2BP1 (ab184305, Abcam), anti-IGF2BP2 (11601-1-AP, Proteintech), anti-IGF2BP3 (ab177477, Abcam), anti-YTHDF1 (ab252346, Abcam), anti-YTHDF3 (ab 220161, Abcam),anti-ACTIN(3700S, CST) and anti-GAPDH(5174S, CST). .. After 3 washes in PBST, the PVDF membranes were incubated with HRP-conjugated goat anti-mouse/rabbit secondary antibodies (1:3000, Boster, China) for 1 h at room temperature.

    Incubation:

    Article Title: METTL3 inhibition alleviates neuroinflammation and apoptosis by reducing ETV4 m6A modification
    Article Snippet: .. Briefly, BV2 cell lysates were incubated with magnetic beads coated with 5 μg of anti-IGF2BP2 (Proteintech, 11601-1-AP) or control mouse IgG (Millipore) antibody for 4 hours at room temperature. ..

    Article Title: TNF-α-driven m6A modification disrupts the immunoregulatory function of MSCs by regulating HDAC5-dependent super-enhancers.
    Article Snippet: SDS‒PAGE was used to separate protein extracts, which were subsequently transferred to 0.45 μm PVDF membranes (Millipore, MA, USA). .. After 1 h of blocking with 5% nonfat milk at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies, including anti-LIF (ab138002, Abcam), antiHDAC5 (ab55403, Abcam), anti-METTL3 (ab195352, Abcam), anti-WTAP (ab195380, Abcam), anti-METTL14 (ab309096, Abcam), anti-ALKBH5 (ab195377, Abcam), anti-FTO (ab126605, Abcam), anti-YTHDF2 (24744-1- AP, Proteintech), anti-YTHDC2 (27779-1-AP, Proteintech), anti-BRD2 (ab139690, Abcam), anti-BRD3 (ab50818, Abcam), anti-BRD4 (ab128874, Abcam), anti-IGF2BP1 (ab184305, Abcam), anti-IGF2BP2 (11601-1-AP, Proteintech), anti-IGF2BP3 (ab177477, Abcam), anti-YTHDF1 (ab252346, Abcam), anti-YTHDF3 (ab 220161, Abcam),anti-ACTIN(3700S, CST) and anti-GAPDH(5174S, CST). .. After 3 washes in PBST, the PVDF membranes were incubated with HRP-conjugated goat anti-mouse/rabbit secondary antibodies (1:3000, Boster, China) for 1 h at room temperature.

    Article Title: TNF-α-driven m6A modification disrupts the immunoregulatory function of MSCs by regulating HDAC5-dependent super-enhancers
    Article Snippet: SDS‒PAGE was used to separate protein extracts, which were subsequently transferred to 0.45 μm PVDF membranes (Millipore, MA, USA). .. After 1 h of blocking with 5% nonfat milk at room temperature, the membranes were incubated overnight at 4 °C with primary antibodies, including anti-LIF (ab138002, Abcam), anti-HDAC5 (ab55403, Abcam), anti-METTL3 (ab195352, Abcam), anti-WTAP (ab195380, Abcam), anti-METTL14 (ab309096, Abcam), anti-ALKBH5 (ab195377, Abcam), anti-FTO (ab126605, Abcam), anti-YTHDF2 (24744-1-AP, Proteintech), anti-YTHDC2 (27779-1-AP, Proteintech), anti-BRD2 (ab139690, Abcam), anti-BRD3 (ab50818, Abcam), anti-BRD4 (ab128874, Abcam), anti-IGF2BP1 (ab184305, Abcam), anti-IGF2BP2 (11601-1-AP, Proteintech), anti-IGF2BP3 (ab177477, Abcam), anti-YTHDF1 (ab252346, Abcam), anti-YTHDF3 (ab 220161, Abcam),anti-ACTIN(3700S, CST) and anti-GAPDH(5174S, CST). .. After 3 washes in PBST, the PVDF membranes were incubated with HRP-conjugated goat anti-mouse/rabbit secondary antibodies (1:3000, Boster, China) for 1 h at room temperature.

    Magnetic Beads:

    Article Title: METTL3 inhibition alleviates neuroinflammation and apoptosis by reducing ETV4 m6A modification
    Article Snippet: .. Briefly, BV2 cell lysates were incubated with magnetic beads coated with 5 μg of anti-IGF2BP2 (Proteintech, 11601-1-AP) or control mouse IgG (Millipore) antibody for 4 hours at room temperature. ..

    Control:

    Article Title: METTL3 inhibition alleviates neuroinflammation and apoptosis by reducing ETV4 m6A modification
    Article Snippet: .. Briefly, BV2 cell lysates were incubated with magnetic beads coated with 5 μg of anti-IGF2BP2 (Proteintech, 11601-1-AP) or control mouse IgG (Millipore) antibody for 4 hours at room temperature. ..

    Staining:

    Article Title: Hilnc- mediated UCP1 translation repression contributes to thermogenesis and energy expenditure
    Article Snippet: .. Proteins were stained with anti-ACTIN (ABclonal, AC038), anti-GFP (Proteintech, 66002-1-IG), anti-UCP1 (Abcam, ab10983-50μL), anti-TOM20 (Proteintech, 11802-1-AP), or anti-IGF2BP2 (Proteintech, 82757-2-RR) overnight at 4 °C, then with HRP-conjugated antibodies of the corresponding species specificities, respectively (anti-rabbit: Invitrogen, 31460; anti-mouse: Invitrogen, 31430). .. Stained membranes were visualized using Enhanced ECL Chemiluminescent Detection Kit (Vazyme, E411-05) with Tanon 5200 Chemiluminescence Imaging System.



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    FTH1 acted as the target of <t>METTL16.</t> A The immunofluorescence assay showed the subcellular localization of METTL16 and FTH1. B The binding motif sites of METTL16 for its target genes (GGAAC). C The FTH1 level in cervical cancer cells was test by the RT-PCR. D RNA immunoprecipitation (RIP) assay was conducted for the binding within METTL16 and FTH1 in HeLa cells. E RIP assay using anti-m 6 A conducted for the m 6 A modification on FTH1 mRNA. * p < 0.05, ** p < 0.01
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    Image Search Results


    FTH1 acted as the target of METTL16. A The immunofluorescence assay showed the subcellular localization of METTL16 and FTH1. B The binding motif sites of METTL16 for its target genes (GGAAC). C The FTH1 level in cervical cancer cells was test by the RT-PCR. D RNA immunoprecipitation (RIP) assay was conducted for the binding within METTL16 and FTH1 in HeLa cells. E RIP assay using anti-m 6 A conducted for the m 6 A modification on FTH1 mRNA. * p < 0.05, ** p < 0.01

    Journal: Discover Oncology

    Article Title: Novel N 6 -methyladenosine (m 6 A) writer METTL16 promotes the cervical cancer tumorigenesis by targeting FTH1-dependent ferroptosis

    doi: 10.1007/s12672-026-04403-8

    Figure Lengend Snippet: FTH1 acted as the target of METTL16. A The immunofluorescence assay showed the subcellular localization of METTL16 and FTH1. B The binding motif sites of METTL16 for its target genes (GGAAC). C The FTH1 level in cervical cancer cells was test by the RT-PCR. D RNA immunoprecipitation (RIP) assay was conducted for the binding within METTL16 and FTH1 in HeLa cells. E RIP assay using anti-m 6 A conducted for the m 6 A modification on FTH1 mRNA. * p < 0.05, ** p < 0.01

    Article Snippet: Cervical cancer cells were lysed by immunoprecipitation buffer (protease, phosphatase inhibitors, MCE) containing magnetic beads conjugated to specific antibodies (anti-IGF2BP2, anti-METTL16, anti-m 6 A).

    Techniques: Immunofluorescence, Binding Assay, Reverse Transcription Polymerase Chain Reaction, RNA Immunoprecipitation, Modification

    IGF2BP2 promoted the stability of FTH1 mRNA. A The immunofluorescent staining showed the subcellular localization of IGF2BP2 and FTH1 in HeLa cells. B The positive correlation within IGF2BP2 and FTH1 in clinical samples ( http://gepia.cancer-pku.cn/index.html ). C RNA immunoprecipitation (RIP) assay showed the immunoprecipitated FTH1 mRNA by anti-IGF2BP2 antibody. D RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with IGF2BP2 overexpression. E RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with METTL16 silencing (sh-METTL16-1, sh-METTL16-2). F RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with IGF2BP2 silencing (si-IGF2BP2) and METTL16 overexpression. * p < 0.05, ** p < 0.01

    Journal: Discover Oncology

    Article Title: Novel N 6 -methyladenosine (m 6 A) writer METTL16 promotes the cervical cancer tumorigenesis by targeting FTH1-dependent ferroptosis

    doi: 10.1007/s12672-026-04403-8

    Figure Lengend Snippet: IGF2BP2 promoted the stability of FTH1 mRNA. A The immunofluorescent staining showed the subcellular localization of IGF2BP2 and FTH1 in HeLa cells. B The positive correlation within IGF2BP2 and FTH1 in clinical samples ( http://gepia.cancer-pku.cn/index.html ). C RNA immunoprecipitation (RIP) assay showed the immunoprecipitated FTH1 mRNA by anti-IGF2BP2 antibody. D RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with IGF2BP2 overexpression. E RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with METTL16 silencing (sh-METTL16-1, sh-METTL16-2). F RNA decay analysis reported the stability of FTH1 mRNA in cervical cancer cells with IGF2BP2 silencing (si-IGF2BP2) and METTL16 overexpression. * p < 0.05, ** p < 0.01

    Article Snippet: Cervical cancer cells were lysed by immunoprecipitation buffer (protease, phosphatase inhibitors, MCE) containing magnetic beads conjugated to specific antibodies (anti-IGF2BP2, anti-METTL16, anti-m 6 A).

    Techniques: Staining, RNA Immunoprecipitation, Immunoprecipitation, Over Expression

    Silencing circGDI2 inhibits proliferation and glycolysis and PKM2 expression through IGF2BP2 in HCC cells. (A) Western blot was used to analyze IGF2BP2 expression in Li-7 and Huh-7 cell lines after circGDI2 knockdown. (B) The expression level of IGF2BP2 was examined in clinical HCC tissues and adjacent normal tissues using RT-qPCR. To clarify if circGDI2 regulated HCC cell proliferation and glycolysis through IGF2BP2, sh-circGDI2 and OE-IGF2BP2 were co-transfected into Li-7 and Huh-7 cells. (C) Western blot was used to assess the effect of IGF2BP2 on the expression of PKM2. (D) CCK-8 assay was used to assess cell proliferation. (E) Glucose consumption level and lactate production were detected using the Glucose Assay Kit with O-toluidine and Lactate Assay Kit. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, vs. the sh-NC group, or the Adjacent group. # P < 0.05, ## P < 0.01, ### P < 0.001, vs. the sh-circGDI2+OE-NC group.

    Journal: Non-coding RNA Research

    Article Title: The regulatory role of circGDI2 in hepatocellular carcinoma proliferation and glycolysis with the involvement of m6A modification

    doi: 10.1016/j.ncrna.2025.11.006

    Figure Lengend Snippet: Silencing circGDI2 inhibits proliferation and glycolysis and PKM2 expression through IGF2BP2 in HCC cells. (A) Western blot was used to analyze IGF2BP2 expression in Li-7 and Huh-7 cell lines after circGDI2 knockdown. (B) The expression level of IGF2BP2 was examined in clinical HCC tissues and adjacent normal tissues using RT-qPCR. To clarify if circGDI2 regulated HCC cell proliferation and glycolysis through IGF2BP2, sh-circGDI2 and OE-IGF2BP2 were co-transfected into Li-7 and Huh-7 cells. (C) Western blot was used to assess the effect of IGF2BP2 on the expression of PKM2. (D) CCK-8 assay was used to assess cell proliferation. (E) Glucose consumption level and lactate production were detected using the Glucose Assay Kit with O-toluidine and Lactate Assay Kit. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, vs. the sh-NC group, or the Adjacent group. # P < 0.05, ## P < 0.01, ### P < 0.001, vs. the sh-circGDI2+OE-NC group.

    Article Snippet: IGF2BP2 , IHC , Rabbit , 1:500 , Proteintech , 11601-1-AP.

    Techniques: Expressing, Western Blot, Knockdown, Quantitative RT-PCR, Transfection, CCK-8 Assay, Glucose Assay, Lactate Assay

    Silencing circGDI2 inhibits HCC tumor growth and PKM2 expression through IGF2BP2. To verify the effect of circGDI2 and IGF2BP2 on HCC tumor growth, a xenograft mouse model was constructed. (A) Pictures of the isolated tumors of the indicated group. (B) The tumor volume and weight were recorded. (C) HE staining, Tunel and Ki-67 staining were performed to observe the histological characteristics and cell proliferation in the tumor tissues (scale bar = 100 μm). (D) RT-qPCR was used to detect circGDI2, IGF2BP2 and PKM2 levels. (E) IHC was used to detect IGF2BP2 and PKM2 levels (scale bar = 100 μm). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, vs. the sh-NC group. # P < 0.05, ## P < 0.01, ### P < 0.001, vs. the sh-circGDI2+OE-NC group.

    Journal: Non-coding RNA Research

    Article Title: The regulatory role of circGDI2 in hepatocellular carcinoma proliferation and glycolysis with the involvement of m6A modification

    doi: 10.1016/j.ncrna.2025.11.006

    Figure Lengend Snippet: Silencing circGDI2 inhibits HCC tumor growth and PKM2 expression through IGF2BP2. To verify the effect of circGDI2 and IGF2BP2 on HCC tumor growth, a xenograft mouse model was constructed. (A) Pictures of the isolated tumors of the indicated group. (B) The tumor volume and weight were recorded. (C) HE staining, Tunel and Ki-67 staining were performed to observe the histological characteristics and cell proliferation in the tumor tissues (scale bar = 100 μm). (D) RT-qPCR was used to detect circGDI2, IGF2BP2 and PKM2 levels. (E) IHC was used to detect IGF2BP2 and PKM2 levels (scale bar = 100 μm). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, vs. the sh-NC group. # P < 0.05, ## P < 0.01, ### P < 0.001, vs. the sh-circGDI2+OE-NC group.

    Article Snippet: IGF2BP2 , IHC , Rabbit , 1:500 , Proteintech , 11601-1-AP.

    Techniques: Expressing, Construct, Isolation, Staining, TUNEL Assay, Quantitative RT-PCR

    Silencing FTO inhibits HCC tumor growth and decreases circRNA, IGF2BP2 and PKM2 levels. To investigate the biological role of FTO on HCC tumor growth, the xenograft tumor models of HCC cells in the sh-NC and sh-FTO groups were established. (A) Pictures of the isolated tumors of the indicated group. (B) The tumor volume and weight were recorded. (C) HE staining, Ki-67 staining and Tunel stainning were performed to observe the histological characteristics and cell proliferation in the tumor tissues (scale bar = 100 μm). (D) RT-qPCR was used to detect circGDI2 and FTO levels. (E) IHC was used to detect IGF2BP2 and PKM2 levels (scale bar = 100 μm). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, vs. the sh-NC group.

    Journal: Non-coding RNA Research

    Article Title: The regulatory role of circGDI2 in hepatocellular carcinoma proliferation and glycolysis with the involvement of m6A modification

    doi: 10.1016/j.ncrna.2025.11.006

    Figure Lengend Snippet: Silencing FTO inhibits HCC tumor growth and decreases circRNA, IGF2BP2 and PKM2 levels. To investigate the biological role of FTO on HCC tumor growth, the xenograft tumor models of HCC cells in the sh-NC and sh-FTO groups were established. (A) Pictures of the isolated tumors of the indicated group. (B) The tumor volume and weight were recorded. (C) HE staining, Ki-67 staining and Tunel stainning were performed to observe the histological characteristics and cell proliferation in the tumor tissues (scale bar = 100 μm). (D) RT-qPCR was used to detect circGDI2 and FTO levels. (E) IHC was used to detect IGF2BP2 and PKM2 levels (scale bar = 100 μm). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, vs. the sh-NC group.

    Article Snippet: IGF2BP2 , IHC , Rabbit , 1:500 , Proteintech , 11601-1-AP.

    Techniques: Isolation, Staining, TUNEL Assay, Quantitative RT-PCR